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human collagen i elisa kit  (Cusabio)


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    Cusabio human collagen i elisa kit
    Human Collagen I Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+col+i+elisa+kit/Human+Collagen+Type+%E2%85%A0%2CCol+%E2%85%A0+ELISA+Kit/pm41599277-135-1-10
    Average 93 stars, based on 14 article reviews
    human collagen i elisa kit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Secretome of human umbilical cord mesenchymal stem cell maintains skin homeostasis by regulating multiple skin physiological function.
    Article Snippet: Skin is the largest organ in the body and the first defense to resist various diseases and external stimuli that easily cause infection and inflammation.. Aseptic inflammation, barrier damage, and foreign aid pressure induce the destruction and damage to the skin microenvironment.. Subsequently, it destroys the skin’s physiological function, leading to the maintenance and circulation of steady-state imbalance and aggravating the process of skin disorders.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Secretome of human umbilical cord mesenchymal stem cell maintains skin homeostasis by regulating multiple skin physiological function.
    Article Snippet: Skin is the largest organ in the body and the first defense to resist various diseases and external stimuli that easily cause infection and inflammation.. Aseptic inflammation, barrier damage, and foreign aid pressure induce the destruction and damage to the skin microenvironment.. Subsequently, it destroys the skin’s physiological function, leading to the maintenance and circulation of steady-state imbalance and aggravating the process of skin disorders.

    Article Title: The Reduction of Skin Photodamage by the Ectoine-Thermus thermophilus Cofermentation Products.
    Article Snippet: .. Human Col I ELISA Kit (CSB- E08082h, Cusabio) and MMP- 1 ELISA kit (KE00223, Proteintech) were utilized to detect the content of Col I and MMP- 1 in the samples. ..

    Article Title: The Reduction of Skin Photodamage by the Ectoine– Thermus thermophilus Cofermentation Products
    Article Snippet: .. Human Col I ELISA Kit (CSB‐E08082h, Cusabio) and MMP‐1 ELISA kit (KE00223, Proteintech) were utilized to detect the content of Col I and MMP‐1 in the samples. ..



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    human collagen i elisa kit  (Cusabio)
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    Cusabio human collagen i elisa kit
    Human Collagen I Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+col+i+elisa+kit/Human+Collagen+Type+%E2%85%A0%2CCol+%E2%85%A0+ELISA+Kit/pm41599277-135-1-10
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    collagen type i  (Cusabio)
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    Inhibition of miR-320a inhibited TGF-β1-induced fibroblast activation . ( A ) The miR-320a inhibition was achieved in TED OFs by transfecting miR-320a inhibitor and verified using qRT-PCR. Then, the TED OFs were transfected with miR-320a inhibitor or inhibitor negative control (NC), exposed to 10 ng/mL TGF-β1 for 48 hours, and conducted a series of experiments. ( B ) Cell viability was detected by CCK-8 assay. ( C ) DNA synthesis was determined using EdU assay. ( D ) The mRNA expression of fibronectin and vimentin were detected using qRT-PCR. ( E ) The protein levels of vimentin and fibronectin were measured using immunoblotting. ( F ) The collagen I content was ascertained using <t>ELISA.</t> ** P < 0.01 versus the blank group; ## P < 0.01 versus the TGF-β1 + inhibitor NC group.
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    Inhibition of miR-320a inhibited TGF-β1-induced fibroblast activation . ( A ) The miR-320a inhibition was achieved in TED OFs by transfecting miR-320a inhibitor and verified using qRT-PCR. Then, the TED OFs were transfected with miR-320a inhibitor or inhibitor negative control (NC), exposed to 10 ng/mL TGF-β1 for 48 hours, and conducted a series of experiments. ( B ) Cell viability was detected by CCK-8 assay. ( C ) DNA synthesis was determined using EdU assay. ( D ) The mRNA expression of fibronectin and vimentin were detected using qRT-PCR. ( E ) The protein levels of vimentin and fibronectin were measured using immunoblotting. ( F ) The collagen I content was ascertained using <t>ELISA.</t> ** P < 0.01 versus the blank group; ## P < 0.01 versus the TGF-β1 + inhibitor NC group.
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    Inhibition of miR-320a inhibited TGF-β1-induced fibroblast activation . ( A ) The miR-320a inhibition was achieved in TED OFs by transfecting miR-320a inhibitor and verified using qRT-PCR. Then, the TED OFs were transfected with miR-320a inhibitor or inhibitor negative control (NC), exposed to 10 ng/mL TGF-β1 for 48 hours, and conducted a series of experiments. ( B ) Cell viability was detected by CCK-8 assay. ( C ) DNA synthesis was determined using EdU assay. ( D ) The mRNA expression of fibronectin and vimentin were detected using qRT-PCR. ( E ) The protein levels of vimentin and fibronectin were measured using immunoblotting. ( F ) The collagen I content was ascertained using <t>ELISA.</t> ** P < 0.01 versus the blank group; ## P < 0.01 versus the TGF-β1 + inhibitor NC group.
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    Inhibition of miR-320a inhibited TGF-β1-induced fibroblast activation . ( A ) The miR-320a inhibition was achieved in TED OFs by transfecting miR-320a inhibitor and verified using qRT-PCR. Then, the TED OFs were transfected with miR-320a inhibitor or inhibitor negative control (NC), exposed to 10 ng/mL TGF-β1 for 48 hours, and conducted a series of experiments. ( B ) Cell viability was detected by CCK-8 assay. ( C ) DNA synthesis was determined using EdU assay. ( D ) The mRNA expression of fibronectin and vimentin were detected using qRT-PCR. ( E ) The protein levels of vimentin and fibronectin were measured using immunoblotting. ( F ) The collagen I content was ascertained using ELISA. ** P < 0.01 versus the blank group; ## P < 0.01 versus the TGF-β1 + inhibitor NC group.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: The miR-320a/PRDX3 Axis Alleviates the Oxidative Stress and Fibrotic Alterations in Fibroblasts in Thyroid Eye Disease

    doi: 10.1167/iovs.66.9.41

    Figure Lengend Snippet: Inhibition of miR-320a inhibited TGF-β1-induced fibroblast activation . ( A ) The miR-320a inhibition was achieved in TED OFs by transfecting miR-320a inhibitor and verified using qRT-PCR. Then, the TED OFs were transfected with miR-320a inhibitor or inhibitor negative control (NC), exposed to 10 ng/mL TGF-β1 for 48 hours, and conducted a series of experiments. ( B ) Cell viability was detected by CCK-8 assay. ( C ) DNA synthesis was determined using EdU assay. ( D ) The mRNA expression of fibronectin and vimentin were detected using qRT-PCR. ( E ) The protein levels of vimentin and fibronectin were measured using immunoblotting. ( F ) The collagen I content was ascertained using ELISA. ** P < 0.01 versus the blank group; ## P < 0.01 versus the TGF-β1 + inhibitor NC group.

    Article Snippet: The secreted Collagen I levels of OFs in culture medium were determined by the Human Collagen Type I ELISA Kit (CSB-E08082h; CUSABIO).

    Techniques: Inhibition, Activation Assay, Quantitative RT-PCR, Transfection, Negative Control, CCK-8 Assay, DNA Synthesis, EdU Assay, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of miR-320a improves the progression of TED in mice . ( A , B ) TED model was established in mice as described; the model was verified using H&E staining examining mice thyroid tissues and ELISA examining serum T4 and TSAb levels. Next, miR-320a inhibition was achieved in mice by orbital injection in TED model mice. ( C ) The photographs of the eyeballs of the model mice were showed. The orbital inflammation in TED mouse model was quantified by a semi-quantitative murine orbital activity score. ( D , E ) histopathological alterations in mice orbital tissues were evaluated using H&E ( D ) and Masson staining ( E ). The collagen volume fraction (CVF) of mice orbital tissues was calculated with ImageJ software. ( F ) The expression levels of miR-320a, vimentin, and fibronectin were determined using qRT-PCR. ( G ) The protein levels of vimentin and fibronectin were determined using immunoblotting. ( H ) The level of collagen I in orbital tissues was determined using ELISA. ** P < 0.01 versus the control group; ## P < 0.01 versus the TED + antagomir-NC group.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: The miR-320a/PRDX3 Axis Alleviates the Oxidative Stress and Fibrotic Alterations in Fibroblasts in Thyroid Eye Disease

    doi: 10.1167/iovs.66.9.41

    Figure Lengend Snippet: Inhibition of miR-320a improves the progression of TED in mice . ( A , B ) TED model was established in mice as described; the model was verified using H&E staining examining mice thyroid tissues and ELISA examining serum T4 and TSAb levels. Next, miR-320a inhibition was achieved in mice by orbital injection in TED model mice. ( C ) The photographs of the eyeballs of the model mice were showed. The orbital inflammation in TED mouse model was quantified by a semi-quantitative murine orbital activity score. ( D , E ) histopathological alterations in mice orbital tissues were evaluated using H&E ( D ) and Masson staining ( E ). The collagen volume fraction (CVF) of mice orbital tissues was calculated with ImageJ software. ( F ) The expression levels of miR-320a, vimentin, and fibronectin were determined using qRT-PCR. ( G ) The protein levels of vimentin and fibronectin were determined using immunoblotting. ( H ) The level of collagen I in orbital tissues was determined using ELISA. ** P < 0.01 versus the control group; ## P < 0.01 versus the TED + antagomir-NC group.

    Article Snippet: The secreted Collagen I levels of OFs in culture medium were determined by the Human Collagen Type I ELISA Kit (CSB-E08082h; CUSABIO).

    Techniques: Inhibition, Staining, Enzyme-linked Immunosorbent Assay, Injection, Activity Assay, Software, Expressing, Quantitative RT-PCR, Western Blot, Control

    Inhibition of PRDX3 partially reversed the inhibitory effects of miR-320a inhibition on fibroblast activation . ( A ) PRDX3 knockdown was achieved in TED OFs by transfecting small interfering RNA (siRNA) against PRDX3 (si-PRDX3#1/#2) and verified using qRT-PCR. Next, TED OFs were co-transfected with si-PRDX3 and miR-320a inhibitor, exposed to 10 ng/mL TGF-β1 for 48 hours, and conducted a series of experiments. ( B ) Cell viability was measured using CCK-8 assay. ( C ) DNA synthesis was detected using EdU assay. ( D ) The mRNA expression levels of fibronectin and vimentin were determined using qRT-PCR. ( E ) The protein levels of vimentin and fibronectin were detected using immunoblotting. ( F ) The collagen I content in culture medium was measured using ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the inhibitor NC + si-NC group; ## P < 0.01 versus the inhibitor NC + si-PRDX3 group; && P < 0.01 versus the miR-320a inhibitor + si-NC group.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: The miR-320a/PRDX3 Axis Alleviates the Oxidative Stress and Fibrotic Alterations in Fibroblasts in Thyroid Eye Disease

    doi: 10.1167/iovs.66.9.41

    Figure Lengend Snippet: Inhibition of PRDX3 partially reversed the inhibitory effects of miR-320a inhibition on fibroblast activation . ( A ) PRDX3 knockdown was achieved in TED OFs by transfecting small interfering RNA (siRNA) against PRDX3 (si-PRDX3#1/#2) and verified using qRT-PCR. Next, TED OFs were co-transfected with si-PRDX3 and miR-320a inhibitor, exposed to 10 ng/mL TGF-β1 for 48 hours, and conducted a series of experiments. ( B ) Cell viability was measured using CCK-8 assay. ( C ) DNA synthesis was detected using EdU assay. ( D ) The mRNA expression levels of fibronectin and vimentin were determined using qRT-PCR. ( E ) The protein levels of vimentin and fibronectin were detected using immunoblotting. ( F ) The collagen I content in culture medium was measured using ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the inhibitor NC + si-NC group; ## P < 0.01 versus the inhibitor NC + si-PRDX3 group; && P < 0.01 versus the miR-320a inhibitor + si-NC group.

    Article Snippet: The secreted Collagen I levels of OFs in culture medium were determined by the Human Collagen Type I ELISA Kit (CSB-E08082h; CUSABIO).

    Techniques: Inhibition, Activation Assay, Knockdown, Small Interfering RNA, Quantitative RT-PCR, Transfection, CCK-8 Assay, DNA Synthesis, EdU Assay, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay